Cholesterol Test Blood System

Cholesterol Test Blood Basics

Cholesterol is a waxy substance that is found in your blood. Your body needs cholesterol to build healthy cells, but high levels of cholesterol can increase your risk of heart disease.
When cholesterol is too high, fatty deposits develop in your blood vessels. Eventually, these deposits grow, making it difficult for enough blood to flow through your arteries. Sometimes these deposits can suddenly rupture and form clots that can lead to a heart attack or stroke.
High cholesterol can be genetic, but it is often the result of unhealthy lifestyle choices, which make it preventable and treatable. A healthy diet, regular exercise, and sometimes medication can help lower high cholesterol.

Causes of High total cholesterol

Cholesterol is carried through the bloodstream and attached to proteins. This combination of protein and cholesterol is called a lipoprotein. There are different types of cholesterol depending on the substances carried by the lipoproteins. They are:

• Low-density lipoproteins (LDL). LDL, or "bad" cholesterol, carries cholesterol particles throughout the body. LDL cholesterol builds up in the walls of your arteries, making them hard and narrow.
• High-density lipoprotein (HDL). HDL, the "good" cholesterol, absorbs the excess cholesterol and brings it back to the liver.

Lipid profiles also usually measure triglycerides, a type of fat in the blood. High triglyceride levels can also increase the risk of heart disease.

Factors you can control - such as inactivity, obesity and an unhealthy diet - can lead to harmful cholesterol and triglyceride levels. Factors beyond your control may also play a role. For example, your genetic makeup may make it more difficult for your body to remove LDL cholesterol from your blood or break it down in your liver.

Conditions that can lead to unhealthy cholesterol levels include

• Chronic kidney disease
• Diabetes
• HIV-AIDS
• Hypothyroidism
• Lupus
• Pregnancy
• Familial hypertcemia (LDL receptor deficiency), familial apolipoprotein b deficiency, mixed hyperlipoproteinemia.

Certain types of medications you may be taking for other health problems can also make cholesterol levels worse, such as

• Acne
• Cancer
• high blood pressure
• HIV AIDS
• Heart arrhythmia
• Organ transplantation

Causes of low HDL

HDL-C is mainly used for risk analysis of atherosclerotic cardiovascular disease, with reduced HDL-C as a risk factor for cardiovascular disease and elevated HDL-C as a protective factor for cardiovascular disease.

Many conditions and lifestyle choices play a role in lowering HDL levels. They include:

• Being overweight: If you are overweight or obese, losing weight can raise HDL levels and lower the risk of heart disease.
• Genetic factors: Sometimes, very low HDL cholesterol levels can be inherited. Conditions that severely lower HDL levels include Tangier's disease and familial low alpha lipoproteinemia. 
• Poor diet: What you eat can also affect your HDL levels. Limit saturated fats (e.g., butter, cream, whole or 2% milk, beef, pork, skin-on chicken) and replace them with monounsaturated and polyunsaturated fats found in olives and avocados.
• Sedentary lifestyle: Adding moderate exercise to your daily routine may slightly improve your HDL levels. Try 40 minutes of aerobic exercise a day, three to four times a week. "Aerobic" includes activities such as swimming, brisk walking, running, cycling and dancing. 
• Smoking: The chemicals in cigarettes can lower your HDL cholesterol. Quitting smoking can help increase your HDL as well as prevent other chronic diseases, including cardiovascular disease.
• Uncontrolled diabetes: High blood sugar (sugar) levels may help lower HDL cholesterol levels. It can also increase triglyceride and LDL levels. Controlling blood sugar may help raise HDL levels

Causes of high triglycerides

High triglycerides (hypertriglyceridemia) can be harmful to your health. Unfortunately, high triglycerides, like high cholesterol, rarely cause symptoms. It is critical to have routine lipid screening to check your cholesterol count.
Your healthcare provider determines total cholesterol by looking at the combination of triglyceride, HDL and LDL numbers. If your triglycerides and LDL cholesterol are high, but your HDL is low, you are at increased risk for heart disease and stroke

To get the most accurate reading, you should fast 8 to 12 hours prior to your lipid test. A healthy value for triglycerides is less than 150 milligrams per deciliter (mg/dL).
Your healthcare provider classifies high triglyceride levels as:
Mild: 150-199 mg/dL.
Moderate: 200-499 mg/dL.
Severe: greater than 500 mg/dL.

Factors that may raise triglyceride levels include

• Excessive alcohol consumption.
• Family history of high cholesterol.
• Liver disease or kidney disease.
• Medications, including diuretics, hormones, corticosteroids, and beta-blockers.
• Menopause.
• Obesity.
• Smoking.
• Thyroid disease.
• Uncontrolled diabetes.
• Diets high in sugar and simple carbohydrates.

Cholesterol Test Blood Testing Principles

The whole blood sample to be tested is added to the spiking zone of the test card. During uniform and rapid downward osmosis, the blood cells are filtered out and the substance to be tested reacts with the enzymes and chemicals in the reaction layer and undergoes a color change that is proportional to the concentration of the substance to be tested. The dry chemistry analyzer detects the color intensity of the reaction at 620 nm and uses this reflection coefficient to calculate the concentrations of total cholesterol, HDL cholesterol and triglycerides.

Cholesterol Test Blood Product Composition

Cholesterol test blood meter
Lipid test strips: (single: TC/TG test strips; three test strips)
Optional: quality control card, blood collection pen, blood collection needle, capillary tube

Cholesterol Meter	Test Device
Safety Lancet	Code Chip
Capillary Transfer Tube/Dropper	Carrying Case
Lancing Device	Sterile Lancets

Cholesterol Test Blood Meter Advantages

The Cholesterol Test Blood meter allows for rapid testing: requires less blood, only 35 microliters (only 10 microliters for a single card), and is less painful and traumatic; results are available in 120 seconds

Accurate results of the cholesterol test blood meter: using the principle of light reflection, the test results are accurate

Cholesterol test blood meter is easy to use: one test can get four results of TC, HDL, TG, LDL; can directly detect a variety of sample types without pre-processing; ultra-wide detection range

Good continuity of cholesterol test blood meter: 1200mAh lithium battery; 300 rechargeable, can be used continuously for about 30 hours (600 measurements) in a full state

Cholesterol test blood meter with large storage: 500 test results can be stored

Cholesterol test blood meter comes with a power saving mode: the analyzer automatically shuts down within 5 minutes without any operation

Cholesterol blood meter can transfer data: versions and records can be transferred to a computer via USB or Bluetooth

Cholesterol Test Blood Technical Parameters and Standards

Measuring range	Total cholesterol(TC) : 2.59-12.93mmol/L H-density lipoprotein cholesterol(HDL-C) : 0.39-2.59mmol/L Triglyceride(TG) : 0.51-7.34mmol/L
Blood sample type	Whole blood, blood serum, and blood plasma
Sample capacity	35ul (three items), 10ul (single item)
MT	< 120S
Storage data	500 Group data and its test date and time and number
Service environment	10-35°C, <80%
Storage environment	0-45°C <90%
Measurement methodology	Photochemistry

Cholesterol Test Blood Operation Procedure

1. After turning on the machine, insert the Code Chip carried with the test strip cartridge into the Code Chip jack on the instrument and check whether the Code Chip number matches the number on the label of the test strip used.

2. Take out a test strip;

3. Insert the test strip into the test strip slot of the instrument in the direction of the arrow on the test strip, with the left and right arrows on the slot aligned with the middle line of the spiked area, and gently push the test strip no longer forward. When the screen shows the blood drop logo, it means that the sample can be added.

4. Insert the test strip into the test strip slot of the instrument in the direction of the arrow of the test strip, with the left and right arrows on the slot aligned with the middle line of the spiked sample area, and gently push the test strip no longer forward. When the screen shows the blood drop logo, it means the sample can be added.

5. After the sample is added, the instrument starts to test automatically. During this test, do not move the instrument or the test strip, and do not press the operation keys on the panel.

6. Within 120 seconds, total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and triglyceride (TG) test values will be displayed; and the concentration value of low-density lipoprotein cholesterol (LDL-C), TC/HDL value will be calculated; the unit can be converted between mmol/L and mg/dL; LDL=TC-HDL-TG/2.2 (mmol/L)

7. After removing the test strip, the screen automatically returns to the interface to be tested.

Cholesterol Test Blood Research Status

Product technical index comparison

Single test strip(electrochemical method)

Vender	BeneCheck	Easy Touch	Redford
test specification	TC： 2.59- 10.35mmol/L	TC： 2.59- 10.35mmol/L	TC： 2.6- 10.4mmol/L
Sample quantity	10ul	4ul	15ul
time	<26s	<150s	<150s
methodology	electrochemistry	electrochemistry	electrochemistry

Lipid triple test strips(Photochemical method）

Vender	Medasia	Wondfo	Mission
Test specification	TC: 2.59- 12.93mmol/L TG: 0.51- 7.34mmol/L HDL: 0.39- 2.59mmol/L	TC: 2.59- 10.36mmol/L TG: 0.57- 6.78mmol/L HDL: 0.52- 2.07mmol/L	TCL 2.59- 12.93mmol/L TGL 0.51- 7.34mmol/L HDL: 0.39- 2.59mmol/L
Sample quantity	35ul	45ul	35pL
time	<120s	<180s	<120s
methodology	electrochemistry	electrochemistry	electrochemistry

 

PTS	SD
TC： 2.59- 10.36mmol/L TG： 0.57-5.65mmol/L HDL： 0.39-2.59mmol/L	TC： 2.59-11.64mmol/L TG： 0.51- 7.34mmol/L HDL： 0.65- 2.46mmol/L
40ul	35pL
<120s	<180s
electrochemistry	electrochemistry

Summary

The Medasia Cholesterol Test Blood System combines dry chemical, enzymatic and colorimetric methods for detection. The detection system uses dry chemistry for optical signal detection. The amount of specimens required is small and the specimens do not need to be processed. The enzyme method is easy to operate, with good specificity and stable results; the photochemical detection method is highly sensitive.

References：

1. Cholesterol test - Mayo Clinic

2. Cholesterol Levels: MedlinePlus Medical Test

See also:

1. Cholesterol care